Spis treści: Green Fluorescent Protein: Properties, Applications and Protocols



First Edition Preface, s. vii
Preface, s. xi
Contributors, s. xiii

1.   DISCOVERY OF GREEN FLUORESCENT PROTEIN / Osamu Shimomura, s. 1

2.   PHOTONS FOR REPORTING MOLECULAR EVENTS: GREEN FLUORESCENT PROTEIN AND FOUR LUCIFERASE SYSTEMS / J. Woodland Hastings and James G. Morin, s. 15

3.   BIOCHEMICAL AND PHYSICAL PROPERTIES OF GREEN FLUORESCENT PROTEIN / William W. Ward, s. 39

4.   THE THREE-DIMENSIONAL STRUCTURE OF GREEN FLUORESCENT PROTEIN AND ITS IMPLICATIONS FOR FUNCTION AND DESIGN / George N. Phillips, Jr., s. 67

5.   MOLECULAR BIOLOGY AND MUTATION OF GREEN FLUORESCENT PROTEIN / David A. Zacharias and Roger Y. Tsien, s. 83

6.   DISCOVERY AND PROPERTIES OF GFP-LIKE PROTEINS FROM NONBIOLUMINESCENT ANTHOZOA / Konstantin A. Lukyanov, Dmitry M. Chudakov, Arkady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, and Sergey Lukyanov, s. 121

7.   EVOLUTION OF FUNCTION AND COLOR IN GFP-LIKE PROTEINS / Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde, s. 139

8.   THE USES OF GREEN FLUORESCENT PROTEIN IN PROKARYOTES / Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow, s. 163

9.   THE USES OF GREEN FLUORESCENT PROTEIN IN YEASTS / Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver, s. 179

10.   USES OF GFP IN CAENORHABDITIS ELEGANS / Oliver Hobert and Paula Loria, s. 203

11.   GREEN FLUORESCENT PROTEIN APPLICATIONS IN DROSOPHILA / Tulle Hazelrigg and Jennifer H. Mansfield, s. 227

12.   THE USES OF GREEN FLUORESCENT PROTEIN IN PLANTS / Jim Haseloff and Kriby R. Siemering, s. 259

13.   USES OF GFP IN TRANSGENIC VERTEBRATES / Sean Magason, Adam Amsterdam, Nancy Hopkins, and Shuo Lin, s. 285

14.   THE USES OF GREEN FLUORESCENT PROTEIN IN MAMMALIAN CELLS / Theresa H. Ward and Jennifer Lippincott-Schwartz, s. 305

15.   PRACTICAL CONSIDERATIONS FOR USE OF REEF CORAL FLUORESCENT PROTEINS IN MAMMALIAN CELLS: APPLICATIONS IN FLUORESCENCE MICROSCOPY AND FLOW CYTOMETRY / Yu Fang, Olivier Dery, Michael Haugwitz, Pierre Turpin, and Steven R. Kain, s. 339

16.  PHARMACEUTICAL APPLICATIONS OF GFP AND RCFP / Nicola Bevan and Stephen Rees, s. 361

17.  REASSEMBLED GFP: DETECTING PROTEIN-PROTEIN INTERACTIONS AND PROTEIN EXPRESSION PATTERNS / Thomas J. Magliery and Lynne Regan, s. 391

Methods and Protocols / Steven R. Kain, s. 407
Index, s. 423
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